Differential Uridyl-diphosphate-Glucuronosyl Transferase 1A enzymatic arsenal explains the specific cytotoxicity of resveratrol towards tumor colorectal cells

Resveratrol belongs to the Bioactive Food Component (BFC) family. It seems admitted that its cytotoxic action impacts tumor cells and spares healthy cells, but published proofs remain rare. We hypothesized may differentially metabolize resveratrol lead different systemic impacts. For this, metabolization was evaluated by ultra-high-performance liquid chromatography (UHPLC) coupled with diode array detection (DAD), correlated expression of Uridyl-diphosphate-Glucuronosyl Transferase 1A (UGT1A) genes. The UGT1A genes in human colorectal tissues studied RNAseq databases. Functional validation enzymes implication sensitivity established modulation. As S phase cell cycle, nucleotide metabolic balance assessed. found more downregulation UGTs, i.e. cells. Conversely, overexpression UGT1A10 gene an initial resveratrol-sensitive line restored accompanied cytotoxicity diminution. affected intestinal sensitive homeostasis a growth/proliferation decoupling, cell-cycle modulation, UXP/AXP imbalance resulting global reduction transcription translation. This impact on activity restricted study improves resveratrol’s general knowledge explains how antitumor can spare non-tumor also paves way select tumors eligible for treatment potentiation without additional toxicity digestive tissues.